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Research into embryogenesis and nervous system
2018-10-22
Research into embryogenesis and nervous system development has been instrumental to the identification of factors required for cell specification. Using information gleaned from these studies, many groups have developed induction protocols to instruct hESCs and iPSCs to become a variety of neural ce
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br Material and methods br Acknowledgment The authors would
2018-10-22
Material and methods Acknowledgment The authors would like to thank Mr. Chee Peng Ng (Institute of Molecular and Cell Biology, Singapore) for his help on the electron scanning microscope and Dr. William Birch (Institute of Materials Research and Engineering, Singapore) for his critical review
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Therefore a major goal remains to understand the complex cel
2018-10-22
Therefore, a major goal remains to understand the complex cellular and molecular network that controls HSC generation and expansion. In fact, HSCs that are mainly slow cycling (Wilson et al., 2008) and reside in the bone marrow during the entire life of an individual, are not generated there. They a
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The increasing number of studies focusing
2018-10-22
The increasing number of studies focusing on regenerative medicine and stem cell transplantation is being accompanied by the development of techniques using many types of nanoparticles. The combination of nanotechnology with stem cell research may be able to address some challenges in this research
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br Results br Discussion In this study
2018-10-22
Results Discussion In this study, we identified altogether 21 novel imprinted DMRs, including a novel class of intergenic DMRs, which reside in regions with no apparent imprinted ABT-263 in PSCs. This class of imprinted DMRs either may regulate genes in a tissue-specific manner, thus adding t
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PLX-4720 Although our work with BRCA mutant iPSCs
2018-10-22
Although our work with BRCA1 mutant iPSCs led to our investigation of PKC-theta upregulation in human breast tumors, future studies will be necessary to validate whether PKC-theta can be used as a biomarker or therapeutic target in BRCA1 mutant tumors. Because the frequency of BRCA1 mutations in pat
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br Materials and methods br Results br Discussion To evaluat
2018-10-22
Materials and methods Results Discussion To evaluate the effects of proNGF on NSC proliferation, we performed cell viability assay, BrdU incorporation assay and neurosphere assay. Our results showed that proNGF significantly reduced the total number of viable cells and the proportion of Brd
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In this study localization of the SSC population
2018-10-22
In this study, localization of the SSC population was determined by PGP 9.5 expression. PGP 9.5, also called ubiquitin C-terminal hydrolase L-1, is expressed in neuronal erk pathway and testes, and specific expression of PGP9.5 was identified in spermatogonia of day 8 and 16 mice, and PGP9.5 express
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br Materials and methods br Results br Discussion
2018-10-20
Materials and methods Results Discussion Strategies aiming at controlled divergence of ASCs to specific differentiated cell types essentially rely on prolonged culture in the presence of specific sets of growth factors and/or matrix proteins (Zuk et al., 2001). Specific culture conditions w
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In this study we were able to successfully delete
2018-10-20
In this study, we were able to successfully delete Klf5 from the Lgr5-expressing intestinal stem ibuprofen msds in adult mice. The short-term effect of Klf5 deletion in the intestinal and colonic stem cells was a gradual disappearance of Klf5 expression starting in the EGFP-positive stem cells and m
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br Acknowledgments We thank all the members of Wagers labora
2018-10-20
Acknowledgments We thank all the members of Wagers laboratory and E. Gussoni and E. Dzierzak for helpful discussions. We also thank A. Wakabayashi, G. Buruzula and J. LaVecchio at Joslin/HSCI Flow Cytometry Core for excellent flow cytometry support, funded in part by NIHP30 DK036836. This work wa
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PDGs possess a specific architecture and molecular
2018-10-20
PDGs possess a specific architecture and molecular signature that make them distinct from the pancreatic epithelium, instead resembling gastric stem cell glands. PDGs were also found to express and up-regulate TFF genes during injury. In the stomach TFF1 is normally found predominantly in the epithe
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In the present study we have used a set
2018-10-20
In the present study, we have used a set of OSCC cell lines and isolated the putative stem cell fraction (CD44highCD24low) along with CD44highCD24high and CD44lowCD24high (non-stem) sub-populations. These populations were subjected to various molecular and cellular assays to determine whether CD24 h
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br Materials and methods br Acknowledgments We thank Dr
2018-10-20
Materials and methods Acknowledgments We thank Dr. Keisuke Okita and Prof. Shinya Yamanaka for providing the reprogramming plasmids. Additionally, we would like to thank Tina Christoffersen and Birgit Kassow for skillful technical assistance. We acknowledge SHARE\'s Cross Faculty PhD Initiativ
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br Materials and methods br Acknowledgments We would
2018-10-20
Materials and methods Acknowledgments We would like to thank Dr. Keisuke Okita and Prof. Shinya Yamanaka for providing the plasmids for reprogramming. We thank Bente Smith Thorup for excellent technical assistance in the cell culture. We thank Mehri Mirrajei for cytogenetic technical assistanc
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