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br Materials and methods br Results br Discussion To evaluat
2018-10-22
Materials and methods Results Discussion To evaluate the effects of proNGF on NSC proliferation, we performed cell viability assay, BrdU incorporation assay and neurosphere assay. Our results showed that proNGF significantly reduced the total number of viable cells and the proportion of Brd
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In this study localization of the SSC population
2018-10-22
In this study, localization of the SSC population was determined by PGP 9.5 expression. PGP 9.5, also called ubiquitin C-terminal hydrolase L-1, is expressed in neuronal erk pathway and testes, and specific expression of PGP9.5 was identified in spermatogonia of day 8 and 16 mice, and PGP9.5 express
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br Materials and methods br Results br Discussion
2018-10-20
Materials and methods Results Discussion Strategies aiming at controlled divergence of ASCs to specific differentiated cell types essentially rely on prolonged culture in the presence of specific sets of growth factors and/or matrix proteins (Zuk et al., 2001). Specific culture conditions w
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In this study we were able to successfully delete
2018-10-20
In this study, we were able to successfully delete Klf5 from the Lgr5-expressing intestinal stem ibuprofen msds in adult mice. The short-term effect of Klf5 deletion in the intestinal and colonic stem cells was a gradual disappearance of Klf5 expression starting in the EGFP-positive stem cells and m
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br Acknowledgments We thank all the members of Wagers labora
2018-10-20
Acknowledgments We thank all the members of Wagers laboratory and E. Gussoni and E. Dzierzak for helpful discussions. We also thank A. Wakabayashi, G. Buruzula and J. LaVecchio at Joslin/HSCI Flow Cytometry Core for excellent flow cytometry support, funded in part by NIHP30 DK036836. This work wa
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PDGs possess a specific architecture and molecular
2018-10-20
PDGs possess a specific architecture and molecular signature that make them distinct from the pancreatic epithelium, instead resembling gastric stem cell glands. PDGs were also found to express and up-regulate TFF genes during injury. In the stomach TFF1 is normally found predominantly in the epithe
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In the present study we have used a set
2018-10-20
In the present study, we have used a set of OSCC cell lines and isolated the putative stem cell fraction (CD44highCD24low) along with CD44highCD24high and CD44lowCD24high (non-stem) sub-populations. These populations were subjected to various molecular and cellular assays to determine whether CD24 h
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br Materials and methods br Acknowledgments We thank Dr
2018-10-20
Materials and methods Acknowledgments We thank Dr. Keisuke Okita and Prof. Shinya Yamanaka for providing the reprogramming plasmids. Additionally, we would like to thank Tina Christoffersen and Birgit Kassow for skillful technical assistance. We acknowledge SHARE\'s Cross Faculty PhD Initiativ
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br Materials and methods br Acknowledgments We would
2018-10-20
Materials and methods Acknowledgments We would like to thank Dr. Keisuke Okita and Prof. Shinya Yamanaka for providing the plasmids for reprogramming. We thank Bente Smith Thorup for excellent technical assistance in the cell culture. We thank Mehri Mirrajei for cytogenetic technical assistanc
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In our current study IGFBP levels were approximately fold
2018-10-20
In our current study IGFBP-2 levels were approximately 10-fold higher than those of IGFBP-3 suggesting that the large induction of IGFBP-2 may play a more import role in the differentiation of DPCs than the decrease seen in IGFBP-3 concentrations. Although an extensive literature describes both IGF-
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br Materials and methods br Author disclosure statement
2018-10-20
Materials and methods Author disclosure statement Acknowledgments This work was supported by the National Institutes of Health K08 grant number HL111148 (J.L.S.) and R01 grant number HL051387 (D.E.V.). We are especially indebted to patients and their families for participating in this study
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Treatment options for xerostomia include administration
2018-10-20
Treatment options for xerostomia include administration of saliva substitutes or stimulants (Fox, 2004). Saliva substitutes might improve some, but not all, problems associated with SG dysfunction, whereas stimulants are only useful for people with some remaining SG function. Alternative approaches
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Although the disease specific phenotype identified in
2018-10-20
Although the disease specific phenotype identified in this study will be invaluable for demonstrating the efficacy of gene based therapeutics, two major questions remain: 1) why do some patients with TRNT1 variants develop the severe syndromic disease SIFD while others (i.e. the three presented in t
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EPZ015666 cost br Introduction Visional signals are
2018-10-20
Introduction Visional signals are mediated by photoreceptors, bipolar EPZ015666 cost and ganglion cells, and the loss of these neurons leads to irreversible blindness (de Jong, 2006; Friedman et al., 2004; Hartong et al., 2006; Quigley and Broman, 2006; Ramsden et al., 2013; Thylefors et al., 19
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In vitro we demonstrated that the DMR can drive luciferase
2018-10-20
In vitro, we demonstrated that the DMR can drive luciferase expression in three different cell lines (293T,COS-7, and HepG2) and markedly enhances reporter gene activity (10- to 15-fold increase) compared with basal activity obtained with either the SIX5 promoter, the entire region that surrounds th
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